Development of Conditions of Submerged Biosynthesis of Landomycin A

Tetiana Todosiichuk, Victor Fedorenko, Maryna Molochko, Oleksandr Gromyko


Background. Development of the process and biotechnology of production of antitumor antibiotic with microbial origin – landomycin A.

Objective. Establishment of the rational parameters for the submerged biosynthesis of landomycin A during the cultivation in the laboratory conditions.

Methods. Cultivation of the producer Streptomyces cyanogenus S136 was performed on shaking devices in the flasks. Antibiotic was extracted from culture fluid with ethyl acetate; the content of antibiotic was determined by measuring optical density of the extract.

Results. Conditions and parameters for antibiotic submerged biosynthesis of the landomycin A by S. cyanogenus S136 were investigated. Alternative nutrient mediums for the biosynthesis of the landomycin A were presented. Mediums that are based on soybean’s flour extract and corn’s flour allow obtaining up to 80 mg/ml of antibiotic. The influence of mixing intensity and temperature on the rate of the product accumulation and its dynamics was established. Rational technological parameters of the producer’s submerged biosynthesis such as temperature 25 ± 1 °С mixing intensity 230–250 U/min, duration of the submerged fermentation 50–60 hours were established.

Conclusions. Variants of the culture media, that were selected (based on the corn’s flour and the extract of the soybean’s flour) and the parameters of the landomycin A biosynthesis, that were set are the basis for optimization and design of the technology for obtaining the antibiotic in industrial conditions.


Streptomyces cyanogenus S136; Biosynthesis; Antibiotic; Landomycin A; Technological parameters


V. Novikov et al., “Trends in commercial biotechnology”, Visn. NAN Ukrayiny, no. 2, pp. 25–39, 2008 (in Ukrainian).

R.V. Kostyuk, “The development of innovative activities of the biotechnological companies in the modern conditions”, Aktual'ni Problemy Ekonomiky, vol. 98, no. 8, pp. 79–84, 2009 (in Ukrainian).

Yu.G. Alyaev et al., “The use of mitomycin in the treatment of scar complications after radical prostatectomy”, Andrologiya i Genitalnaya Hirurgiya, no. 2, pp. 19–25, 2013 (in Russian).

A.P. Viktorov et al., “Modern antineoplastic drugs: problems”, Zdorov’ya Ukrayiny, April, pp. 26–28, 2010 (in Russian).

B. Ostash et al., “Chemistry and biology of landomycins, an expanding family of polyketide natural products”, Mini-Rev. Med. Chem., vol. 9, no. 9, pp. 1040–1051, 2009. doi: 10.2174/138955709788922593

M. Oskay, “Antifungal and antibacterial compounds from Streptomyces strains”, African J. Biotechnol., vol. 8, no. 13, pp. 3007–3017, 2009.

Yucel et al., “Selection of Streptomyces isolates from Turkish karstic caves against antibiotic resistant microorganisms”, Pak. J. Pharm., vol. 23, pp. 1–6, 2010.

S. Sanchez et al., “Metabolic regulation of fermentation process”, Enzyme Microb Technol., vol. 31, no. 7, pp. 895–906, 2002. doi: 10.1016/S0141-0229(02)00172-2

R. Rafieenia, “Effect of nutrients and culture conditions on antibiotic synthesis”, Asian J. Pharm. Hea. Sci., vol. 3, pp. 810–815, 2013.

S. Sarkar, “Enhanced antimicrobials production by Streptomyces sundarbansensis sp. nov. in a novel extended surface biofilm reactor”, Int. J. Adv. Biotechnol. Res., vol. 6, no. 1, pp. 12–20, 2015.

S. Dutta et al., “Kinetics of rapamycin production by Streptomyces hygroscopicus MTCC 4003”, 3 Biotech., vol. 4, pp. 523–531, 2014. doi: 10.1007/s13205-013-0189-2

Y. Liaoa et al., “Effect of fermentation temperature on validamycin A production by Streptomyces hygroscopicus 5008”, J. Bio­technol., vol. 142, no. 3-4, pp. 271–274, 2009. doi: 10.1016/j.jbiotec.2009.04.015

M. Kumari et al., “Identification and characterization of antibiotic producing actinomycetes isolates”, Am. J. Microbiol., vol. 4, no. 1, pp. 24–31, 2013. doi: 10.3844/ajmsp.2013.24.31

S. Bundale et al., “Optimization of culture conditions for production of bioactive metabolites by Streptomyces spp. isolated from soil”, Advances in Microbiology, vol. 5, pp. 441–451, 2015. doi: 10.4236/aim.2015.56045

A.M. Luzhets'kyy et al., “A method of production landomycin A”, UA Patent 84 651, Oct. 25, 2013 (in Ukrainian).

GOST Style Citations





  • There are currently no refbacks.

Copyright (c) 2017 NTUU KPI