Antimicrobial Action of Compositions Based on Thiosulfonates and Biosurfactants on Phytopathogens
Background. The development of environmentally safe preparations with antimicrobial activity on microorganism-phytopathogens.
Objective. Determination of antimicrobial activity of compositions alilthiosulfonate (ATS) and metylthiosulfonate (MTS) with surface-active rhamnolipid biocomplex (RBC) on phytopathogens Agrobacterium tumefaciens and Сlavibacter michiganensis.
Methods. Investigation in vitro of antimicrobial activity of compositions based on thiosulfonates and rhamnolipid biocomplex on microorganism-phytopathogens A. tumefaciens and C. michiganensis in accordance with the minimum inhibitory concentration and minimum bactericidic concentrations of developed compositions. Studies of biosurfactant effect on permeability of cell membraines.
Results. It is shown that the composition of methyl- and alilthiosulfanilates with RBC are more activе and have lower values of minimum inhibitory and bactericidic concentrations than ATS and MTS alone – on average by 50 %.
Conclusions. The results showed the prospects of rhamnolipid-biosurfactants use for creation of highly effective compositions with biocides for plant protection.
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S. Hojgard and K. Faruk, “Antibiotic resistance. Why is the problem so difficult to solve?”, Infect. Ecol. Epidemiol., vol. 7, no. 14, pp. 1113–1124, 2012. doi: 10.3402/iee.v2i0.18165
V.І. Lubenets, “Thiosulfonates: synthesis and properties”, Ukrayins'kyy Khimichnyy Zhurnal, vol. 69, no. 3, pp. 109–117, 2003 (in Ukrainian).
V. Lubenets et al., “Synthesis and antimicrobial properties of 4-acylaminobenzenethiosulfoacid S-esters”, Saudi Pharmaceutical Journal, vol. 25, no. 2, pp. 266–274, 2017. doi: 10.1016/j.jsps.2016.06.007
B. Reddy et al., “Chemopreventive effect of S-methylmethane thiosulfonate and sulindac administered together during the promotion/progression stages of colon carcinogenesis”, Carcinogenesis, vol. 20, no. 8, pp. 1645–1648, 1999. doi: 10.1093/carcin/20.8.1645
D. Galabova et al., “Permeabilization of Yarrowia lipolytica cells by triton X-100”, Enzyme and Microbial Technology, vol. 18, pp. 18–22, 1996. doi: 10.1016/0141-0229(96)00063-4
M. Pacwa-Płociniczak et al., “Environmental applications of biosurfactants: recent advances”, Int. J. Mol. Sci., vol. 12, pp. 633–654, 2011. doi: 10.3390/ijms12010633
V. Lubenets et al., “Development of new antimicrobial compositions of thiosulfonate structure”, Chemistry and Chemical Technology, vol. 7, no. 2, pp. 119–124, 2013.
A. Sotirova et al., “The importance of rhamnolipid-biosurfactant induced changes in bacterial membrane lipids of Bacillus subtilis for the antimicrobial activity of thiosulfonates”, Current Microbiol., vol. 65, pp. 534–541, 2012. doi: 10.1007/s00284-012-0191-7
V. Lubenets et al., “The plant protection remedies of thiosulfonate type”, Chem. Agricult. Environ., vol. 8, pp. 163–167, 2007.
E.V. Karpenko et al., “Optimal methods for isolating biogenic surface-active rhamnolipids”, Zhurnal Obshhej Himii, vol. 12, pp. 2011–2014, 2009 (in Russian).
J. Segy, Methods of Soil Microbiology. Moscow, Russia: Kolos, 1983 (in Russian).
D.P. Sachdev and S.S. Cameotra, “Biosurfactants in agriculture”, Appl. Microbiol. Biotechnol., vol. 97, pp. 1005–1016, 2013. doi: 10.1007/s00253-012-4641-8
A. Sotirova et al., “Rhamnolipid-biosurfactant permeabilizing effects on Gram-positive and Gram-negative bacterial strains”, Current Microbiol., vol. 56, pp. 639–644, 2008. doi: 10.1007/s00284-008-9139-3
A. Sotirova et al., “Antimicrobial potential of selected thiosulfonates-based biocides and biosurfactants against bacteria and fungi”, Reports BAS. Biologie Microbiologie, vol. 63, no. 9, pp. 1307–1314, 2010.
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