The Biosynthesis of Antibiotics as a Taxonomic Marker of Some Species of the Genus Pseudomonas
DOI:
https://doi.org/10.20535/1810-0546.2017.3.87281Keywords:
Species of the genus Pseudomonas, Antibiotics, TaxonomyAbstract
Background. The ability to biosynthesis of antibiotics was established for 20 of 250 species of Pseudomonas. The value of the biosynthesis of antibiotics as a taxonomic characteristic for the genus Pseudomonas is not studied. This issue will be analyzed using the experimental results.
Оbjective. The aim of the paper is to identify the ability of usage of antibiotics synthesized by certain types of bacteria of the genus Pseudomonas, as a taxonomic marker.
Methods. Methods of thin-layer chromatography (TLC) and LC/MS-analysis were used to determine the synthesis of antibiotics.
Results. A unique set of antibiotics was determined for some types of bacteria Pseudomonas, which are specific for them only: pyocyanin – P. aeruginosa, 2,4-diacetylphloroglucinol – P. brassicacearum, 2-hydroxyphenazine-1-carboxylic acid – P. chlororaphis subsp. aureofaciens, pyrrolnitrin and 2,4-diacetylphloroglucinol – P. protegens, batumin – P. batumici.
Conclusions. The ability to synthesize certain antibiotic substances may serve as a taxonomic marker and be used to identify certain species of Pseudomonas. However, the biosynthesis of antibiotics did not show specificity for several species. This problem requires a deeper study of different types of Pseudomonas.
References
N. Palleroni, “The Pseudomonas story”, Environ. Microbiol., vol. 6, no. 12, pp. 1377–1383, 2010. doi:10.1111/j.1462-2920.2009.02041
A. Peix et al., “Historical evolution and current status of the taxonomy of genus Pseudomonas”, Infection, Genetics and Evolution, vol. 9, pp. 1132–1147, 2009. doi:10.1016/j.meegid.2009.08.001
H. Bernd et al., Pseudomonas: Model Organism, Pathogen, Cell Factory. Verlag GmbH: Wiley-VCH, 2008. doi: 10.1002/9783527622009.
V.V. Smirnov and E.A. Kiprianova, Bacteria of the Genus Pseudomonas. Kyiv, Ukraine: Naukova Dumka, 1990 (in Russian).
L.N. Churkina et al., “Pseudomonas batumici IMV В-7532 – the antibiotic-producing strain”, UA Patent 107822 , June 24, 2016 (in Ukrainian).
E.A. Kiprianova et al., “Pseudomonas batumici sp. nov., the antibiotic-producing bacteria isolated from soil of the Caucasus Black Sea coast”, Microbiol. Zhurnal, vol. 73, no. 5, pp. 3–8, 2011.
S. Leland et al., “Metabolism and function of phenazines in bacteria: impacts on the behavior of bacteria in the environment and biotechnological processes”, Appl. Microbiol. Biotechnol., no. 86, pp. 1659–1670. 2010. doi: 10.1007/s00253-010-2509-3
Microbial Phenazines, S. Chincholkar and L. Thomashow, eds. Springer-Verlag Berlin: Heidelberg, 2013. doi: 10.1007/978-3-642-40573-0
A. Ramette et al., “Pseudomonas protegens sp. nov., widespread plant-protecting bacteria producing the biocontrol compounds 2,4-diacetylphloroglucinol and pyoluteorin”, Syst. Appl. Microbiol., no. 34, pp. 180–188, 2011. doi: 10.1016/j.syapm.2010.10.005
Downloads
Published
Issue
Section
License
Copyright (c) 2017 NTUU KPI Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work
