Biogenic Rhamnolipid Surfactants in Complex Plant Growth Regulators

Authors

  • Ілона Василівна Карпенко Department of Physical Chemistry of Fossil Fuels of the Institute of Physical Organic Chemistry and Coal Chemistry, National Academy of Sciences of Ukraine, Ukraine https://orcid.org/0000-0002-6464-8792
  • Галина Григорівна Мідяна Department of Physical Chemistry of Fossil Fuels of the Institute of Physical Organic Chemistry and Coal Chemistry, National Academy of Sciences of Ukraine, Ukraine https://orcid.org/0000-0003-2328-1020
  • Олена Володимирівна Карпенко Department of Physical Chemistry of Fossil Fuels of the Institute of Physical Organic Chemistry and Coal Chemistry, National Academy of Sciences of Ukraine, Ukraine https://orcid.org/0000-0002-1943-8673

DOI:

https://doi.org/10.20535/1810-0546.2016.3.65576

Keywords:

Rhamnolipid surfactants, Coleoptile, Hypocotyl, Cytokinin, Gibberellic acid, Auxin

Abstract

Background. The topical problem of biotechnology is the creation of ecologically safe agents for the crop production. Considering the environmental threat of synthetic agents, biological products are priority ones for plants. Among them biogenic surfactants deserve attention due to their physico-chemical and biological properties.

Objective. Influence of surface-active products of microbial synthesis of the strain Pseudomonas sp. PS-17 (rhamnolipid biosurfactants) on the activity of phytohormones for the creation of complex plant growth regulators.

Methods. The effect of culture liquid supernatant (CLS) and rhamnolipid biocomplex (RBC) on phytohormone activity was determined in bioassays for auxin, cytokinins, gibberellins.

Results. It was shown that in the compositions with RBC the activity of phytohormones has increased: auxins – indolyl-3-acetic acid on 28 %, indolyl-3-butyric acid – 63 %; gibberellin – on 30 %, cytokinin – on 24–30 % compared to the options without biosurfactants. Such stimulating effect on phytohormones was also determined for CLS, in addition it manifested phytohormone-like action.

Conclusions. The obtained results prove the prospects of rhamnolipid surfactants for the creation of ecologically safe complex plant growth regulators.

Author Biographies

Ілона Василівна Карпенко, Department of Physical Chemistry of Fossil Fuels of the Institute of Physical Organic Chemistry and Coal Chemistry, National Academy of Sciences of Ukraine

Ilona V. Karpenko,

PhD student

Галина Григорівна Мідяна, Department of Physical Chemistry of Fossil Fuels of the Institute of Physical Organic Chemistry and Coal Chemistry, National Academy of Sciences of Ukraine

Galyna G. Midyana,

PhD, chief of department

Олена Володимирівна Карпенко, Department of Physical Chemistry of Fossil Fuels of the Institute of Physical Organic Chemistry and Coal Chemistry, National Academy of Sciences of Ukraine

Olena V. Karpenko,

ScD, head of Division of Chemistry and Biotechnology

References

I.V. Karpenko et al., “Influence of biogenic surfactants on the oil seeds growth”, Visnyk Natsionalnoho Universytetu “Lviska politekhnika”. Ser. Khimiia, Tekhnolohiia Rechovyn ta Yikh Zastosuvannia, no. 787, pp. 254–257, 2014 (in Ukrainian).

O.V. Karpenko et al., “Stimulation of the growth of Gramineae plants by rhamnolipid surfactants”, Biotechnologia Acta, no. 6, pp. 94–99, 2013 (in Ukrainian).

A. Costacurta et al., “Synthesis of phytohormones by plant-associated bacteria”, Crit. Rev. Microbiol., vol. 21, pp. 1–18, 1995.

R.M. Rivero et al., “Cytokinin-dependent photorespiration and the protection of photosynthesis during water deficit”, Plant Physiol., vol. 150, no. 3, pp. 1530–1540, 2009.

R.M. Rivero et al., “Delayed leaf senescence induces extreme drought tolerance in a flowering plant”, Proc. Natl. Acad. Sci. USA, vol. 104, no. 49, pp. 19631–19636, 2007.

M. Iqbal et al., “Gibberellic acid mediated induction of salt tolerance in wheat plants: Growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis”, Environ. Exp. Bot., vol. 86, pp. 76–85, 2013.

D.P. Sachdev et al., “Biosurfactants in agriculture”, Appl. Microbiol. Biotech., vol. 97, pp. 1005–1016, 2013.

L. Chrzanowski et al., “Rhamnolipid biosurfactants decrease the toxicity of chlorinated phenols to Pseudomonas putida DOT-T1E”, Lett. Appl. Microbiol., vol. 48, pp. 756–762, 2009.

E. Deziel et al., “RhlA is required for the production of a novel biosurfactant promoting swarming motility in Pseudomonas aeruginosa: 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs), the precursors of rhamnolipids”, Microbiology, vol. 149, pp. 2005–2013, 2003.

M.E. Davey et al., “Rhamnolipid surfactant production affects biofilms architecture in Pseudomonas aeruginosa PA-01”, J. Bacteriology, vol. 185, pp. 1027–1036, 2003.

V.I. Kefeli et al., Methods for Determination of Plant Hormones, Growth Inhibitors, Defoliants and Herbicides. Moscow, USSR: Kolos, 1973 (in Russian).

L.V. Kharkhota et al., “Rhizogeny of woody stem cuttings of the ornamental less-common shrubs in Donbass”, Promyslova Botanika, iss. 8, pp. 161–165, 2008 (in Ukrainian).

K.B. Evensen et al., “Differences in endogenous levels of gibberellin-like substances in nodules of Phaseolus lunatus L. plants inoculated with two Rhizobium strains”, Plant Physiol., vol. 68. pp. 195–198, 1981.

A.A. Shlyk, “Determination of chlorophylls and carotenoids in extracts of green leaves”, in Biochemical Methods in Plant Physiology. Moscow, USSR: Nauka, 1971, pp. 154–170 (in Russian).

P.S. Richard et al., “Selective delipidation of the plasma membrane by surfactants enrichment of sterols and activation of ATPase”, Plant Physiol., vol. 90, no. 4, pp. 1524–1531, 1989.

E.V. Shumilina et al., “The influence of surfactants on the activity of DII phospholipase”, Biologicheskie Membrany, vol. 15, no. 4. pp. 414–419, 1998 (in Russian).

В. Joseph et al., “Characterization of plant growth promoting rhozobacteria associated with chickpea (Cicer arietinum L.)”, Int. J. Plant Production, vol. 1, no. 2, pp. 141–152, 2007.

K.H. Isayeva, “Formation of gibberellin and gibberellin-like substances by hydrocarbon-oxidizing bacteria”, Vestnik Moskovskogo Gosudarstvennogo Oblastnogo Universiteta. Ser. Estestvennye Nauki, no. 4. pp. 96–101, 2009 (in Russian).

I.A. Tarchevsky, “Pigment content as an indicator of the development power of the photosynthetic apparatus of wheat”, Fiziologija Rastenij, vol. 2. pp. 341–348, 1980 (in Russian).

G. Ouzounidou, “Hormone-induced protection of sunflower photosynthetic apparatus against copper toxicity”, Biol. Plant., vol. 49, pp. 223–228, 2005.

Published

2016-06-30