The Pullulanase Enzyme: Analysis of Industry Use of Producers, a Process for Preparing

Марина Вікторівна Молочко, Наталія Віталіївна Дехтяренко

Abstract


Вackground. The work is dedicated to critical analysis of literature covering the main aspects of pullulanasе enzymes.

Оbjective. The aim is to determine promising areas of research in the field of pullulanasе enzymes.

Methods. The analysis and synthesis of information about the use, sources, mechanism of action, pullulanase enzyme properties and methods of cultivation and methods of isolation and purification of enzyme, immobilization techniques and methods of determining the activity of the enzyme were made.

Results. The analysis of the literature identified the existence of a large number of laboratory developments receiving pullulanase enzyme and lack of industrial production, both in our country and in neighboring countries.

Conclusions. A list of areas in which there is the successful use of pullulanase enzyme is outlined. A patent search of the contemporary producers of pullulanase was made, and characteristics of their cultivation were identified. The most common ways to obtain purified pullulanase and modern methods of immobilization have been revealed. Methods of determining the activity of pullulanase enzyme relatively pullulan substrate were established. Pullulanase enzymes word producers range is specified.


Keywords


Pullulanase; Enzyme; Industry; Mechanism of action; Producer; Property; Activity; Purification; Manufacturers; Strain

References


C. Velhal et al., “Production of pullulanase using novel organic substrates”, Int. J. Sci. Technol. Res., no. 3, pp. 94–97, 2014.

N.І. Sabadash, “Improving technology high maltose syrups from corn starch”, Ph.D. thesis, National University of Food Technology, Ukraine, 2009 (in Ukrainian).

R. Malakar et al., “Pullulanase: a potential enzyme for industrial application”, Int. J. Biomed. Res., no. 2, pp. 10–20, 2010.

N.I. Sabadash, “Method of manufacturing a maltose syrup”, Ukraine Patent 57627, IPC S13K 7/00, July 15, 2010 (in Ukrainian).

A.S. Zakirova et al., “Enzymatic modification of the amylopectin”, Vestnik Kazanskogo Tehnologicheskogo Universiteta, no. 7, pp. 164–167, 2013 (in Russian).

I.M. Hodulyan, “Relevance of using enzymes to improve the colloidal stability of beer under the modernized production using unmalted raw materials”, in Sci. Works Collection of the Odessa National Academy of Food Technologies, pp. 12–13, 2014 (in Ukrainian).

The Comprehensive Enzyme Information System [Online]. Available: http://www.brenda-enzymes.info

N.V. Tsurikova, “The strain Bacillus licheniformis bacteria – producing complex thermostable amylolytic and proteolytic enzymes”, Russian Federation Patent 2177995, IPC C12N1/20, C12N9/28, C12N9/44, C12N9/56, C12N1/20, C12R1:10, Feb. 5, 1998 (in Russian).

F. Duffner et al., “A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis”, J. Bacteriology, no. 22, pp. 6331–6338, 2000.

W.T. Ismaya et al. (2012). Chromatography as the Major Tool in the Identification and the Structure-Function Relationship Study of Amylolytic Enzymes from Saccharomycopsis Fibuligera R64 [Online]. Available: http://www.intechopen.com/books/ chromatography-the-most-versatile-method-of-chemical-analysis

G.S. Drummond et al., “Mechanism of action of pullulanase”, FEBS Lett., no. 5, pp. 85–88, 1969.

O.Yu. Galkin, “Modern theoretical methods of studying the antigenic structure of proteins”, Visnyk Zaporiz'koho Natsional'noho Universytetutu. Ser. Biolohichni Nauky, no. 3, pp. 69–77, 2013 (in Ukrainian).

I.A. Krivova, “Development of a biotechnological process of obtaining comprehensive pullulanase enzyme preparation and its use for starch industry”, Ph.D. thesis, Moscow State University of Food Production, Russia, 2006 (in Russian).

M. Waleed et al., “Production optimization of pullulanase enzyme produced by Bacillus cereus isolated from Syrian sources”, Int. Food Res. J., no. 5, pp. 1824–1830, 2015.

A. Wasko et al., “Purification and characterization of pullulanase from Lactococcus lactis”, Prep. Biochem. Biotechnol., no. 4, pp. 73–78, 2011.

D. Malle et al., “Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168”, Structural Biology and Crystallization Commun., no. 4, pp. 381–384, 2006.

J. Long et al., “New method for the immobilization of pullulanase onto hybrid magnetic (Fe3O4-κ-carrageenan) nanoparticles by electrostatic coupling with pullulanase/chitosan complex”, J. Agricultural Food Chem., no. 13, pp. 34–42, 2015.

A. Dessouki et al., “Pullulanase immobilization on natural and synthetic polymers”, J. Technol. Biotechnol., no. 7, pp. 700–706, 2001.

C. Pedersen, “The process of starch processing”, Russian Federation Patent 2315811, IPC C12P19/22, C12P19/20, C12P19/18, C12P19/14, Feb. 10, 2003 (in Russian).

The Official Website of Novozymes [Online]. Avaliable: http://www.novozymes.com


GOST Style Citations


  1. Velhal C., Sant M., Das S. Production of pullulanase using novel organic substrates // Int. J. Sci. Technol. Res. – 2014. – № 3. – Р. 94–97.

  2. Сабадаш Н.І. Удосконалення технології високомальтозних сиропів із кукурудзяного крохмалю: Aвтореф. дис. … к.т.н: спец. 05.18.05 “Харчова технологія”. – К., 2009. – 20 с.

  3. Malakar R., Tiwari A., Malviya S. Pullulanase: a potential enzyme for industrial application // Int. J. Biomed. Res. – 2010. – № 2. – Р. 10–20.

  4. Спосіб виробництва мальтозних сиропів: Пат. 57627 Україна, МПК С13К 7/00 / Сабадаш Н.І.; власник Національний університет харчових технологій. – № 201008828; Заявл. 15.07.2010; Опубл. 10.03.2011, Бюл. № 5.

  5. Закирова А.Ш., Канарский А.В., Канарская З.А. Ферментативная модификация амилопектина // Вестник Казанского технолог. ун-та. – 2013. – № 7. – С. 164–167.

  6. Годулян І.М. Актуальність використання ферментних препаратів для підвищення колоїдної стабільності пива в умовах модернізованого виробництва з використанням несоложеної сировини // Зб. наук. праць молодих учених, аспірантів та студентів. – Одеса: Одеська нац. академія харчових технологій, 2014. – С. 12–13.

  7. База даних “The Comprehensive Enzyme Information System” [Електронний ресурс]. – URL: http://www.brenda-enzy­mes.info

  8. Штамм бактерий Bacillus licheniformis – продуцент комплекса термостабильных амилолитических и протеолитических ферментов: Пат. 2177995 РФ, МПК C12N1/20, C12N9/28, C12N9/44, C12N9/56, C12N1/20, C12R1:10 / Н.В. Цурикова; заявитель и патентообладатель Научно-производственная компания “Фермтек”. – № 98102082/13; Заявл. 05.02.98; Опубл. 10.01.02, Бюл. № 1.

  9. Duffner F., Bertoldo C., Andersen J.T. A new thermoactive pullulanase from Desulfurococcus mucosus: cloning, sequencing, purification, and characterization of the recombinant enzyme after expression in Bacillus subtilis // J. Bacteriology. – 2000. – № 22. – Р. 6331–6338.

  10. Ismaya W.T., Hasan K., Subroto T. Chromatography as the major tool in the identification and the structure-function relationship study of amylolytic enzymes from Saccharomycopsis fibuligera R64 [Online] // Chromatography – The Most Versatile Method of Chemical Analysis. – 2012. – URL: http://www.intechopen.com/books/chromatography-the-most-versatile-method-of-chemical-analysis

  11. Drummond G.S., Smith E.E., Whelan W.J. Mechanism of action of pullulanase // FEBS Lett. – 1969. – № 5. – Р. 85–88.

  12. Галкін О.Ю. Сучасні теоретичні методи вивчення антигенної структури білків // Вісник Запорізького нац. ун-ту. Сер. Біологічні науки. – 2013. – № 3. – С. 69–77.

  13. Кривова И.А. Разработка биотехнологического процесса получения комплексного ферментного препарата пуллуланазы и использование его для крахмалопаточной промышленности: Автореф. дисс. … канд. биолог. наук: спец. 03.00.04 “Биохимия”. – М., 2006. – 28 с.

  14. Waleed M., Faiza A.A., Nizar I. Production optimization of pullulanase enzyme produced by Bacillus cereus isolated from Syrian sources // Int. Food Res. J. – 2015. – № 5. – Р. 1824–1830.

  15. Wasko A., Polak-Berecka M., Targonski Z. Purification and characterization of pullulanase from Lactococcus lactis // Prep. Biochem. Biotechnol. – 2011. – № 4. – Р. 73–78.

  16. Malle D., Itoh T., Hashimoto W. Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168 // Structural Biology and Crystallization Commun. – 2006. – № 4. – Р. 381–384.

  17. Long J., Wu Z., Li X. New method for the immobilization of pullulanase onto hybrid magnetic (Fe3O4-κ-carrageenan) nanoparticles by electrostatic coupling with pullulanase/chitosan complex // J. Agricultural Food Chem. – 2015. – № 13. – Р. 34–42.

  18. Dessouki A., Issa G., Atia K. Pullulanase immobilization on natural and synthetic polymers // J. Technol. Biotechnol. – 2001. – № 7. – Р. 700–706.

  19. Способ обработки крахмала: Пат. 2315811 РФ, МПК C12P19/22, C12P19/20, C12P19/18, C12P19/14 / С. Педерсен; заявитель и патентообладатель Новозимс А/С. – № 20098348/11; Заявл. 10.02.03; Опубл. 27.01.08, Бюл. № 3.

  20. Офіційний сайт компанії Novozymes [Електронний ресурс]. – URL: http://www.novozymes.com




DOI: https://doi.org/10.20535/1810-0546.2016.3.67428

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