The Pullulanase Enzyme: Analysis of Industry Use of Producers, a Process for Preparing
DOI:
https://doi.org/10.20535/1810-0546.2016.3.67428Keywords:
Pullulanase, Enzyme, Industry, Mechanism of action, Producer, Property, Activity, Purification, Manufacturers, StrainAbstract
Вackground. The work is dedicated to critical analysis of literature covering the main aspects of pullulanasе enzymes.
Оbjective. The aim is to determine promising areas of research in the field of pullulanasе enzymes.
Methods. The analysis and synthesis of information about the use, sources, mechanism of action, pullulanase enzyme properties and methods of cultivation and methods of isolation and purification of enzyme, immobilization techniques and methods of determining the activity of the enzyme were made.
Results. The analysis of the literature identified the existence of a large number of laboratory developments receiving pullulanase enzyme and lack of industrial production, both in our country and in neighboring countries.
Conclusions. A list of areas in which there is the successful use of pullulanase enzyme is outlined. A patent search of the contemporary producers of pullulanase was made, and characteristics of their cultivation were identified. The most common ways to obtain purified pullulanase and modern methods of immobilization have been revealed. Methods of determining the activity of pullulanase enzyme relatively pullulan substrate were established. Pullulanase enzymes word producers range is specified.
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