Antiviral and Interferon-Inducing Action of Aminoethoxydiphenyls

Олена Віталіївна Шемендюк, Лілія Григорівна Жолнер, Надія Михайлівна Жолобак, Микола Якович Співак

Abstract


Background. Synthesized compounds 4,4¢-bis(2-R-ethoxy)diphenyl characterized as intercalation into DNA and antiviral activity and ability to induce interferon.

Objective. Determine the antiviral activity and interferon inducer actions of diphenyl derivatives – analogues of Amiksin, in terms of in vitro.

Methods. The work on cell cultures PST and MDBK in conditions in vitro studied antiviral and interferon inducer of the new structural analogues og Amiksin: 4,4¢-bis-(2-dimethylamino-ethoxy)diphenyl, 4,4¢-bis-(2-4-metylpiperydyno-ethoxy)diphenyl and 4,4¢-bis-(2-[2-methyl-2-(4-metylpiperazyn-1-yl)-propil]amino-ethoxy)diphenyl.

Results. Diphenyl derivatives shown the ability to inhibit the development of viral cytopathic effect on the model PST-BBC as in prophylactic and therapeutic regimens in compounds. In the model of inoculated cell cultures PST shown that compounds like Amiksin capable of inducing interferon, their advantage is much less toxicity. With the introduction of experimental animals experimental compounds IFN gene-active substances have been better than Amiksin.

Conclusions The results can be considered as derivatives of diphenyl compounds that are able to provide virus protection cells not only due to the activation of interferon production, but other mechanisms, the study of which is the goal of future research.


Keywords


Amiksin; Antiviral activity; Vesicular stomatitis virus; Interferon inducing action; Diphenyl derivatives

References


N.Ya. Spivak et al., “Antibacterial efficacy of interferon and its inducers”, Mikrobiologicheskij Zhurnal, vol. 61, no. 1, pp. 32–46, 1999 (in Russian).

D.S. Shay et al., “Interferongene activity of analogues of amiksan and derivatives of biphenyl”, Mikrobiologicheskij Zhurnal, vol. 69, no. 5, pp. 59–64, 2007 (in Ukrainian).

A. Wacker et al., “Distribution of 14C-Tilorone in mice”, Naturwissenschaften, no. 59, p. 520, 1972.

Tiloronum [Online]. Avaliable: https://ru.wikipedia.org/wiki/%D0%A2%D0%B8%D0%BB%D0%BE%D1%80%D0%BE% D0%BD.

S.A. Zanoza et al., “Synthesis and cytotoxicity of aminoetoksydyfenily”, Zhurnal Orhanichnoyi ta Farmatsevtychnoyi Khimiyi, vol. 12, no. 3, pp. 38–44, 2014 (in Ukrainian).

M.O. Shibinskaya et al., “Synthesis, cytotoxicity, antiviral activity and interferon inducing ability of 6-(2-aminoethyl)-6h-indolo[2,3-b]quinoxalines”, Europ. J. Med. Chem., vol. 45, no. 3, pp. 1237–1243, 2010.

F.I. Ershov and O.I. Kiselev, Interferons and their Inducers (from Molecules to Drugs). Moscow, Russia: GOETAR-Media, 2005, 368 p. (in Russian).

A.E. Medvedev et al., “A study of the action of immunosuppressive factors from tumour cells on lymphocytes and macrophages in vitro and on the graft-versus-host reaction in mice”, Biomed. Sci., vol. 1, no. 3, pp. 261–266, 1990.

L.N. Lazarenko et al., HPV Infection and Interferon System. Kyiv, Ukraine: Fitosotsiotsentr, 2008, pp. 237–238 (in Ukrainian).

D.A. Novikov and V.V. Novochadov, Statistical Methods in Biomedical Experiments (Typically). Volgograd, Russia: VSMU, 2005, 84 p. (in Russian).


GOST Style Citations


  1. Антибактериальная эффективность препаратов интерферона и его индукторов / Н.Я. Спивак, Н.И. Грабченко, Л.Н. Ла¬заренко и др. // Микробиол. журн. – 1999. – 61, № 1. – C. 32–46.

  2. Інтерфероногенна активність аналогів аміксину і похідних дифенілу / Д.С. Шай, Н.М. Жолобак, С.А. Ляхов та ін. // Мікробіол. журнал. – 2007. – 69, № 5. – С. 59–64.

  3. Wacker A., Lodemann E., Gaur V. Distribution of 14C-Tilorone in mice // Naturwis-senschaften. – 1972. – № 59. – P. 520.

  4. Тилорон [Электронный ресурс]. – Режим доступа: https://ru.wikipedia.org/wiki/ %D0%A2%D0%B8%D0%BB% D0%BE%D1% 80%D0%BE%D0%BD.

  5. Синтез і цитотоксичність аміноетоксидифенілів / С.О. Заноза, Г.В. Мальцев, С.А. Ляхов та ін. // Журнал органічної та фармацевтичної хімії. – 2014. – 12, № 3. – С. 38–44.

  6. Synthesis, cytotoxicity, antiviral activity and interferon inducing ability of 6-(2-aminoethyl)-6h-indolo[2,3-b]quinoxalines / M.O. Shibinskaya, A.V. Mazepa, S.A. Andronati et al. // Europ. J. Med. Chem. – 2010. – 45, № 3. – Р.1237–1243.

  7. Ершов Ф. И., Киселев О.И. Интерфероны и их индукторы (от молекул до лекарств). – М.: ГОЭТАР-Медиа, 2005. – 368 с.

  8. A study of the action of immunosuppressive factors from tumour cells on lymphocytes and macrophages in vitro and on the graft-versus-host reaction in mice / A.E. Medvedev, В.B. Fuchs, A.L. Rankhmilevich et al. // Biomed. Sci. – 1990. – 1, № 3. – P. 261–266.

  9. Папилломавирусная инфекция и система интерферона / Л.Н. Лазаренко, Н.Я. Спивак, О.М. Михайленко и др. – К.: Фитосоциоцентр, 2008. – С. 237–238.

  10. Новиков Д.А., Новочадов В.В. Статистические методы в медико-биологическом эксперименте (типовые случаи). – Волгоград: Изд-во ВолГМУ, 2005. – 84 с.




DOI: http://dx.doi.org/10.20535/1810-0546.2015.3.61925

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