Study of Antiviral Activity of Recombinant Human Interleukin-7 with Various Experimental Models of Hepatitis C Viral Infection

Юлія Іванівна Іванівна Порва, Світлана Леонтіївна Рибалко, Світлана Терентіївна Дядюн, Тетяна Миколаївна Луценко, Олександр Юрійович Галкін, Яніна Олександрівна Похоленко, Оксана Борисівна Горбатюк

Abstract


Background. Interleukin-7 (IL-7) is one of the most important regulatory cytokine of immune system. Given the ability of IL-7 to the modulation of T- and B-cell responses and T-cell homeostasis we may assume that IL-7 not only has the ability to influence the formation of specific immunity and immunodeficiency state, but also inhibit the reproduction of viruses, including hepatitis C virus (HCV).

Objective. Study of antiviral activity of recombinant human IL-7 (rIL-7) with experimental models of viral hepatitis C infection in vitro on sensitive virus epithelial and lymphoid cells.

Methods. We have used the following inoculated cell cultures: Jurkat, rat neurinoma of gasserian ganglion and bovine kidney. As used surrogate HCV we used virus of bovine diarrhea. To study the antiviral activity different concentrations of rIL-7 were injected into the cell culture, producing HCV, and determined virus load. Also we have performed cytological analysis of cells and determined its proliferative activity under influence of rIL-7.

Results. It has been shown that rIL-7 inhibits surrogate HCV reproduction in in vitro conditions (SS50 – 3 mg/ml, ED50 – 4.7 ng/ml, IS – 640). Highest proliferation of intact T-cells is determined at rIL-7 doses 0.3 mg/m and 0.025 mg/ml. rIL-7 affected HCV infected cells differently: during the first 3 days the number of cells decreased or did not change, and after 2–3 weeks the number of cells increased almost 2 times. When we injected rIL-7 with dose of 6 mg/ml within 3 days we obtained 89% viral inhibition at the 3rd day and 100 % at the 4th day; using the dose of rIL-7 0.3 mg/ml the inhibition on the 4th day was 100 %; using dose of rIL-7 1.5 mg/ml the inhibition settled at 55 % for 4 days.

Conclusions. As a result of the studies directed towards determining the effect of rIL-7 on surrogate HCV reproduction, HCV cDNA producing transfected human T-cells Jurkat, it was showed that rIL-7 effectively inhibits virus reproduction.

 


Keywords


Human interleukin-7 recombinant; Hepatitis C virus; Antiviral activity; Viral load

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DOI: http://dx.doi.org/10.20535/1810-0546.2015.3.61735

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