Сultivation Features of Higher Basidiomycetes Trametes zonatus for Liquid Medium

Лариса Олександрівна Тітова, Інна Рішардівна Клечак

Abstract


Background. The basidiomycetes T. zonatus are producers and cellulose oxidative enzymes and polysaccharides with antitumor activity. The feasibility of this study is that the creation of functional food biotechnology from strains of biomass from domestic collections is not enough initial data: fragmentary data on cultivation conditions and culture medium composition, biochemical biomass of T. zonatus.

Оbjective. The aim of the study is to conduct screening of basidiomycetes strains T. zonatus liquid culture medium to determine the effect of the culture medium and the initial pH value of the concentration of biomass T. zonatus 5302, to study the amino acid composition of biomass obtained by submerged T. zonatus 5302 on an culture medium.

Methods. Objects of the research: thirteen basidiomycetes strains T. zonatus IBC. The Standard methods submerged and surface cultivation, conditions cultivation chosen for this strains. Biomass weight was determined by content of dry matter, in the culture broth – areometer method. The source of carbon and nitrogen added to the medium in an amount equimolar respectively to 20 g/dm3 glucose in terms of carbon and 3 g/dm3 NaNO3 in terms of nitrogen. Amino acid composition of proteins after appropriate treatment was studied using amino acid analyzer T-339 (“Mikrotechna”, Czech Republic).

Results. According to the results of screening the best strain found by growth characteristics T. zonatus 5302: concentration of biomass – 4.5 g/dm3 productivity biosynthesis biomass – 0.6 g/dm3·day, the economic growth coefficient – 47 %. For culture medium T. zonatus 5302 selected the source of carbon, nitrogen, growth factors, initial pH culture medium.

Conclusions. The biomass T. zonatus 5302, got by submerged cultivation, in the proposed complex culture medium is characterized by a high content of tyrosine and methionine. Index biological value protein strain T. zonatus 5302 amounted to 0.59, which is equal to those of fungi such as Pleurotus ostreatus (0.52) and Ganoderma lucidum (0.48).



Keywords


Basidiomycetes; Тrametes zonatus; Screening; Submerged cultivation; Medium formulation; Biomass

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DOI: https://doi.org/10.20535/1810-0546.2015.3.60717

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