Influence of Nitrogen Sources on Biomass Growth of Microalgae Chlorella vulgaris

Наталія Борисівна Голуб, Ігор Ігорович Левтун, Єлізавета Дмитрівна Тимошенко

Abstract


Background. Microalgae is perspective resource for biodiesel fuel production. However there is no clear data about the best nitrogen source and its contents for high biomass yield and high lipid contents in it.

Objective. Determination of nitrogen sources influence on Chlorella vulgaris biomass yield for its usage as a raw material in biofuels obtaining process.

Methods. Cultivation of microalgae was conducted using mediums containing: CO(NH2)2, NH4NO3, NH4Cl and КNO3as the nitrogen source. The biomass growth dynamics measurements were based on optical density determination method.

Results. The highest biomass yield occurred in the culture grown with NH4Cl in the medium, and a bit lower yield levels where observed in the medium containing urea, that supports the theory, which stated that more energy is needed to transform nitrogen into restored form. Consumption of ammonium ions leads to rapid decrease of pH level of the medium down to 5. The usage of urea allows keeping medium pH on the level of 7.5.

Conclusions. For maximum yield of Chlorella vulgaris biomass it is necessary to use different nitrogen sources, that allow controlling pH in cultivation process.



Keywords


Microalgae; Chlorella vulgaris; Nitrogen source; Biomass growth; Biofuel

References


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GOST Style Citations


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DOI: https://doi.org/10.20535/1810-0546.2015.3.60707

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