Selection of Methods for Carotenoid Extraction from Submerged Laetiporus sulphureus Mycelium
DOI:
https://doi.org/10.20535/1810-0546.2016.3.60515Keywords:
Carotenoids, Xanthоcarotene, Extraction, Optical density, Submerged cultivation, Laetiporus sulphureusAbstract
Background. To obtainnatural carotenoids for their practical application is the actual problem. Xanthоcarotene obtained through biotechnology can be used in light industry as dye, as an alternative to artificial pigments. The need of dedication of natural carotenoidsis also caused by their suitability for medical use. It is explained by diverse biological activity (antitumor, radioprotective, antimicrobial, antiviral, antioxidant) of these substances.
Objective. Objective of the study is selection of effective methods and conditions of carotenoid extraction from Laetiporus sulphureus biomass.
Methods. After the submerged producer’s cultivation was performed different methods of pigments extraction were carried out. Carotenoid content was determined.
Results. The most effective extraction was chosen. Carotenoid content in producer’s biomass was calculated.
Conclusions. Еthanol-hexane method was selected as the most effective extraction circuit. Also was found that the change of exposure’s temperature causes changes in the composition of the key faction.
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