The Effect of Irradiation by Blue Range of the Spectrum Light on Increasing of Transient Accumulation of Recombinant Reporter Protein GFP in Plants Nicotiana benthamiana
Background. Plant expression systems are being increasingly used in scientific and industrial biotechnology. Research of the influence of different factors on efficiency of plant transient expression systems expands scientific basis for large-scale and economically efficient application of these systems in industrial biotechnology.
Objective. The object of this research was to determine the influence of Irradiation by blue light of plants Nicotiana benthamiana immediately before agroinfiltration on transient accumulation of reporter recombinant protein GFP.
Methods. Irradiation of plants was performed using LED light sources (λ = 440 nm). Quantity of accumulated reporter protein in plant leaves was measured by fluorimetric method.
Results. It was demonstrated that GFP accumulation in irradiated plant biomass was higher than in not irradiated biomass. Increase of level of accumulation was between 60–87 % depending on other agroinfiltration conditions.
Conclusions. Shown effect seems to be promising as a methodological procedure either in labs or in industrial practices. That is because the apparatus for irradiation is constructively simple, cheap, energy efficient and a period of irradiation time is short.
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GOST Style Citations
- Optimization and utilization of Agrobacterium-mediated transient protein production in Nicotiana / M. Shamloul, J. Trusa, V. Mett, V. Yusibov // J. Visualized Experiments. – 2014. – 86. – P. 1–13. doi:10.3791/51204
- Sheludko Y.V. Agrobacterium-mediated transient expression as an approach to production of recombinant proteins in plants // Recent Patents on Biotechnology. – 2008. – 2, № 3. – P. 198–208.
- Gleba Y. Biotech development concepts: plant made pharmaceuticals // CBR Biotech Strategies. – Berlin, May 20, 2014. – 46 p.
- Kaiser J. Is drought over for pharming? // Science. – 2008. – 320, iss. 5875. – P. 473–475. doi:10.1126/science.320.5875.473
- Winans S.C. Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolic compounds, phosphate starvation, and acidic growth media // J. Bacteriol. – 1990. – 172, № 5. – P. 2433–2438.
- Cho H., Winans S.C. VirA and VirG activate the Ti plasmid repABC operon, elevating plasmid copy number in response to wound-released chemical signals // PNAS. – 2005. – 102, № 41. – P. 14843–14848.
- Simmons C.W., Vander Gheynst J.S., Upadhyaya S.K.. A model of Agrobacterium tumefaciens vacuum infiltration into harvested leaf tissue and subsequent in planta transgene transient expression // Biotechnol. Bioeng. – 2009. – 102. – P. 965–970. doi:10.1002/bit.22118
- Melotto M., Underwood W., He S.Y. Role of stomata in plant innate immunity and foliar bacterial diseases // Annu. Rev. Phytopathol. – 2008. – 46. – P. 101–122. doi:10.1146/annurev.phyto.121107.104959
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- Wang Y., Noguchi K., Terashima I. Distinct light responses of the adaxial and abaxial stomata in intact leaves of Helianthus annuus L. // Plant, Cell and Environment. – 2008. – 31. – P. 1307–1316. doi:10.1111/j.1365-3040.2008.01843.x
- Pemadasa M.A. Movements of abaxial and adaxial stomata // Nezv Phytol. – 1979. – 82. – P. 69–80.
- Raschke K. Saturation kinetics of the velocity of stomatal closing in response to CO2 // Plant Physiol. – 1972. – 49. – P. 229–234.
- Plant stomata function in innate immunity against bacterial invasion / M. Melotto, W. Underwood, J. Koczan et al. // Cell. – 2006. – 126. – P. 969–980. doi:10.1016/j.cell.2006.06.054
- Systemic Agrobacterium tumefaciens – mediated transfection of viral replicons for efficient transient expression in plants / S. Marillonnet, C. Thoeringer, R. Kandzia et al. // Nature Biotechnology. – 2005. – 23, № 6. – P. 718–723. doi:10.1038/nbt1094
- Canopy light and plant health / C.L. Ballare, C.A. Mazza, A. Austin, R. Pierik // Plant Physiology. – 2012. – 160. – P. 145–155. doi:10.1104/pp.112.200733
- Biological systems of the host cell involved in Agrobacterium infection / V. Citovsky, S.V. Kozlovsky, B. Lacroix et al. // Cellular Microbiology. – 2007. – 9, № 1. – P. 9–20. doi:10.1111/j.1462-5822.2006.00830.x
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