The Effect of Irradiation by Blue Range of the Spectrum Light on Increasing of Transient Accumulation of Recombinant Reporter Protein GFP in Plants Nicotiana benthamiana

Антон Андрійович Петерсон, Максим Юрійович Василенко, Юлія Сергіївна Прищепа, Лариса Борисівна Орябінська, Микола Вікторович Кучук

Abstract


Background. Plant expression systems are being increasingly used in scientific and industrial biotechnology. Research of the influence of different factors on efficiency of plant transient expression systems expands scientific basis for large-scale and economically efficient application of these systems in industrial biotechnology.

Objective. The object of this research was to determine the influence of Irradiation by blue light of plants Nicotiana benthamiana immediately before agroinfiltration on transient accumulation of reporter recombinant protein GFP.

Methods. Irradiation of plants was performed using LED light sources (λ = 440 nm). Quantity of accumulated reporter protein in plant leaves was measured by fluorimetric method.

Results. It was demonstrated that GFP accumulation in irradiated plant biomass was higher than in not irradiated biomass. Increase of level of accumulation was between 60–87 % depending on other agroinfiltration conditions.

Conclusions. Shown effect seems to be promising as a methodological procedure either in labs or in industrial practices. That is because the apparatus for irradiation is constructively simple, cheap, energy efficient and a period of irradiation time is short.


Keywords


Plant expression system; Transient expression; Nicotiana benthamiana; Recombinant protein; Green fluorescent protein; GFP

References


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GOST Style Citations


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  3. Gleba Y. Biotech development concepts: plant made pharmaceuticals // CBR Biotech Strategies. – Berlin, May 20, 2014. – 46 p.

  4. Kaiser J. Is drought over for pharming? // Science. – 2008. – 320, iss. 5875. – P. 473–475. doi:10.1126/science.320.5875.473

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DOI: https://doi.org/10.20535/1810-0546.2016.3.60469

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