Detection of Allelic Variants of Wax Gene among National and Foreign Barley Varieties

Authors

  • Олена Василівна Степаненко Institute of cell biology and genetic engineering of the NASU, Ukraine
  • Богдан Володимирович Моргун Institute of cell biology and genetic engineering of the NASU, Ukraine
  • Олександр Ілліч Рибалка Plant Breeding and Genetics – National Center of Seed & Cultivar Invastigation of the UAAS, Ukraine
  • Антон Ігорович Степаненко Institute of cell biology and genetic engineering of the NASU, Ukraine
  • Євгеній Васильович Кузьмінський NTUU KPI, Ukraine

DOI:

https://doi.org/10.20535/1810-0546.2014.3.53524

Keywords:

Barley (Hordeum vulgare L.), Wax genes, PCR, Molecular markers, Starch

Abstract

The objective of the present study was to apply of the molecular marker system for detection of the allelic variants of the Wаx gene. Starch pasting properties are very important factors in malting, food processing and feed quality of barley cultivars. Therefore, it is important to control of the amylose/amylopectin ratio in the barley starch. The Wаx locus of barley (Hordeum vulgare L.) is responsible for the amylose endosperm biosynthesis. The Wаx gene may be found among the barley cultivars in several allelic variants: 2 functional active alleles encoding GBSS I protein and one null-allele with the inactivated enzyme.The most reliable way to assess the allelic state of Wаx gene is to use molecular marker based on polymerase chain reaction. Among the 126 barley samples studied (cultivars and breeding lines) by a codominant molecular marker system all of the three allelic variants of Wаx gene were identified. Two samples representing with the cultivars Candle and Alamo carried the null-allele of the Wаx gene. The results of the present study can be efficiently used to identify genotypes with the null-allele of the Wаx gene for developing and characterization of the new barley varieties.

Author Biographies

Олена Василівна Степаненко, Institute of cell biology and genetic engineering of the NASU

Stepanenko Olena V., first category engineer, student at the NTUU KPI

Богдан Володимирович Моргун, Institute of cell biology and genetic engineering of the NASU

Morgun Bogdan V., candidate of sciences (biology), senior research fellow

Олександр Ілліч Рибалка, Plant Breeding and Genetics – National Center of Seed & Cultivar Invastigation of the UAAS

Rybalka Oleksandr I., doctor of biological sciences, leading research fellow, head of department

Антон Ігорович Степаненко, Institute of cell biology and genetic engineering of the NASU

Stepanenko Anton І., leading engineer, postgraduate student at the NTUU KPI

Євгеній Васильович Кузьмінський, NTUU KPI

Kuzminskyi Evhenii V., doctor of chemistry, full professor, head of department

References

M. James and K. Denyer, “Starch synthesis in the cereal endosperm”, Curr. Opin. Plant Biol., vol. 6, pp. 215–222, 2003.

A. Regina et al., “High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats”, Proc. Natl. Acad. Sci. USA., vol. 103, pp. 3546–3551, 2006.

Копусь М.М., Игнатьева Н.Г., Васюшкина Н.Е. та ін. Генетический полиморфизм амилолитических ферментов зерна пшеницы и генетика ферментов биосинтеза крахмала // Зерн. хоз. России. – 2009. – № 4. – С. 23–27.

N. Ishikawaet al., “Artificial induction and characterization of amylose-free mutants of barley”, Barley Genet. Newsl., vol. 24, pp. 49–53, 1995.

E. Domon et al., “The insertion/deletion polymorphisms in the wаxy gene of barley genetic resources from East Asia”, Theor. Appl. Genet., vol. 104, pp. 132–138, 2002.

Кирдогло Е.К., Полищук С.С., Червонис М.В. Методология и результаты селекции ячменя пищевого ис-пользования // Тр. по прикл. ботанике, генетике и селекции. – 2013. – 171. – C. 240–253.

Яковишин Л.А. Избранные главы биоорганической химии. – Севастополь: Стрижак-пресс, 2006. – 196 с.

Метрологiчна характеристика методу спектрофотометричного визначення вмiсту амілози в крохмалi зерна селекцiйних лiнiй пшеницi / I.В. Петрова, О.М. Хохлов, С.В. Чеботар, Ю.М. Сиволап // Фізіологія і біохімія культ. рослин. – 2010. – 42, № 2. – С. 146–152.

M. Somma, “Extraction and purification of DNA. Session 4”, іn Training Course on the Analisis of Food Samples for the Presence of Genetically Modified Organisms. User Manual, M. Querci, M. Jermini, Eds. Luxembourg: DJ joint Research Centre, Institute for Health and Consumer Protection, p. 229, 2006.

J.R. Brody and S.E. Kern, “History and principles of conductive media for standard DNA electrophoresis”, Anal. Biochem., vol. 333, pp. 1–13, 2004.

Downloads

Published

2015-11-14

Issue

No. 3 (2014): Chemical and Biological Sciences and Technologies

Section

Art

License

Copyright (c) 2017 NTUU KPI Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work