Detection of Allelic Variants of Wax Gene among National and Foreign Barley Varieties

Олена Василівна Степаненко, Богдан Володимирович Моргун, Олександр Ілліч Рибалка, Антон Ігорович Степаненко, Євгеній Васильович Кузьмінський

Abstract


The objective of the present study was to apply of the molecular marker system for detection of the allelic variants of the Wаx gene. Starch pasting properties are very important factors in malting, food processing and feed quality of barley cultivars. Therefore, it is important to control of the amylose/amylopectin ratio in the barley starch. The Wаx locus of barley (Hordeum vulgare L.) is responsible for the amylose endosperm biosynthesis. The Wаx gene may be found among the barley cultivars in several allelic variants: 2 functional active alleles encoding GBSS I protein and one null-allele with the inactivated enzyme.The most reliable way to assess the allelic state of Wаx gene is to use molecular marker based on polymerase chain reaction. Among the 126 barley samples studied (cultivars and breeding lines) by a codominant molecular marker system all of the three allelic variants of Wаx gene were identified. Two samples representing with the cultivars Candle and Alamo carried the null-allele of the Wаx gene. The results of the present study can be efficiently used to identify genotypes with the null-allele of the Wаx gene for developing and characterization of the new barley varieties.


Keywords


Barley (Hordeum vulgare L.); Wax genes; PCR; Molecular markers; Starch

References


M. James and K. Denyer, “Starch synthesis in the cereal endosperm”, Curr. Opin. Plant Biol., vol. 6, pp. 215–222, 2003.

A. Regina et al., “High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats”, Proc. Natl. Acad. Sci. USA., vol. 103, pp. 3546–3551, 2006.

Копусь М.М., Игнатьева Н.Г., Васюшкина Н.Е. та ін. Генетический полиморфизм амилолитических ферментов зерна пшеницы и генетика ферментов биосинтеза крахмала // Зерн. хоз. России. – 2009. – № 4. – С. 23–27.

N. Ishikawaet al., “Artificial induction and characterization of amylose-free mutants of barley”, Barley Genet. Newsl., vol. 24, pp. 49–53, 1995.

E. Domon et al., “The insertion/deletion polymorphisms in the wаxy gene of barley genetic resources from East Asia”, Theor. Appl. Genet., vol. 104, pp. 132–138, 2002.

Кирдогло Е.К., Полищук С.С., Червонис М.В. Методология и результаты селекции ячменя пищевого ис-пользования // Тр. по прикл. ботанике, генетике и селекции. – 2013. – 171. – C. 240–253.

Яковишин Л.А. Избранные главы биоорганической химии. – Севастополь: Стрижак-пресс, 2006. – 196 с.

Метрологiчна характеристика методу спектрофотометричного визначення вмiсту амілози в крохмалi зерна селекцiйних лiнiй пшеницi / I.В. Петрова, О.М. Хохлов, С.В. Чеботар, Ю.М. Сиволап // Фізіологія і біохімія культ. рослин. – 2010. – 42, № 2. – С. 146–152.

M. Somma, “Extraction and purification of DNA. Session 4”, іn Training Course on the Analisis of Food Samples for the Presence of Genetically Modified Organisms. User Manual, M. Querci, M. Jermini, Eds. Luxembourg: DJ joint Research Centre, Institute for Health and Consumer Protection, p. 229, 2006.

J.R. Brody and S.E. Kern, “History and principles of conductive media for standard DNA electrophoresis”, Anal. Biochem., vol. 333, pp. 1–13, 2004.


GOST Style Citations






DOI: https://doi.org/10.20535/1810-0546.2014.3.53524

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 NTUU KPI