Methaneproducing of Mesophilous Bacteria Communities Obtained from Livestock Wastes

Людмила Анатоліївна Хрокало, Анна Iгорівна Доломан, Володимир Юлійович Черненко

Abstract


Background. The perspective substrates for elaboration of biopreparations in biogas technologies were considered.

Objective. The aim of the study is selection from farm wastes (pig manure, chicken excrements and fermented farm wastes) storage cultures of mesophilous bacteria communities, comparative analysis of methane producing, selection and identification of methane-producing bacteria cultures.

Methods. Bacteria cultures were grown in anaerobic conditions on liquid and solid Zhylina nutrient media with addition of sodium acetate under temperature +33 ± 2 °C. Content of gaseous metabolites was estimated by using gas chromatograph LHM-8MD. Culture patterns were taken in argon flow, were fixed, Gram stained and viewed in light microscope.

Results. All selected storage cultures efficiently produced methane, about 10–15 vol. % of methane has been accumulated in bottles since the 12th day of cultivation. Сultures selected from fermented wastes had high yields of methane emission is about 28 vol. %. Cultures of methane-producing bacteria were received by passages on liquid and solid nutrient media with addition of amoxicillin. Bacteria of Methanosarcina and Methanosaeta genus were identified.

Conclutions. Obtained cultures are perspective ones for elaboration of bacterial preparations and their further using in biogas technologies.


Keywords


Anaerobic mesophilous bacteria communities; Methane-producing bacteria; Methanosarcina; Methanosaeta

References


M. Mruzek and B. Groda, “Analysis of biogas production from grass silage, depending on its quality”, Acta Univ. Agric. et Silvic. Mendel. Brun., vol. 59, no. 6, pp. 239–246, 2011.

A. Schnurer and A. Jarvis, “Microbiological handbook for biogas plants”, SGG, Uppsala, Swedish Gas Centre Report 207, 2010, 138 p.

W. Song et al., “Cogeneration of hydrogen and methane from protein-mixed food waste by two-phase anaerobic process”, Int. J. Hydrogen Energy, vol. 35, pp. 3141–3146, 2010.

F. Passos et al., “Preatreatment of microalgae to improve biogas production: a review”, Bioresour. Technol, vol. 172, pp. 403–412, 2014.

A. Ward et al., “Anaerobic digestion of algae biomass: a review”, Algal Res, vol. 5, рр. 205–212, 2014.

T.N. Zhilina and G.A. Zavarzin, Methods of Selection and Cultivation of Methane-Forming Bacteria. Puschino, USSR, 1978, 75 р. (in Russian).

R.S. Wolfe et al., “Technigues for cultivation methanogens”, in Methods of Enzymology, A. Rosenzweig and S. Ragsdale, Eds. Salt Lake: Academic Press, pp. 1–22, 2011.

R.S. Wolfe and W.W. Metcalf, “A vacuum-vortex technigue for preparation of anoxic solution of liquid culture media in small volumes for cultivation methanogens of other strict anaerobes”, Anaerobe, vol. 16, no. 3, pp. 216–225, 2010.

Bergey’s Manual of Systematic Bacteriology. 1. The Archaea and the Deeply Branching, and Phototrophic Bacteria, D.R. Boone et al., Eds., 2nd ed. Springer, 2001, 721 p.

V.І. Каrpenko et al., “Interaction of microbial populations in methanogenic communities and directions of methane emission increasing in methane-tanks”, Visnyk Dnipropetrovskogo Universytetu. Ser. Biologia. Ecologia, vol. 1, iss. 14, no. 3, pp. 80−85, 2006 (in Ukrainian).

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GOST Style Citations


  1. Mruzek M., Groda B. Analysis of biogas production from grass silage, depending on its quality // Acta Univ. Agric. et Silvic. Mendel. Brun. – 2011. – 59, № 6. – Р. 239–246.

  2. Schnurer A., Jarvis A. Microbiological Handbook for Biogas Plants: Swedish Gas Centre Report 207. – Uppsala: SGG, 2010. – 138 p.

  3. Cogeneration of hydrogen and methane from protein-mixed food waste by two-phase anaerobic process / W. Song, J. Cheng, J. Zhou et al. // Int. J. Hydrogen Energy. – 2010. – 35. – P. 3141–3146.

  4. Preatreatment of microalgae to improve biogas production: a review / F. Passos, E. Uggetti, H. Carrere, I. Ferrer // Bioresour. Technol. – 2014. – 172. – P. 403–412.

  5. Ward A., Lewis D., Green F. Anaerobic digestion of algae biomass: a review // Algal Res. – 2014. – 5. – P. 205–212.

  6. Жилина Т.Н., Заварзин Г.А. Методы выделения и культивирования метанообразующих бактерій. – Пущино, 1978. – 75 с.

  7. Wolfe R.S., Amy C.R., Stephen W.R. Technigues for cultivation methanogens // Methods of Enzymology / A. Rosenzweig, S. Ragsdale, Eds. – Salt Lake: Academic Press, 2011. – P. 1–22.

  8. Wolfe R.S., Metcalf W.W. A vacuum-vortex technigue for preparation of anoxic solution of liquid culture media in small volumes for cultivation methanogens of other strict anaerobes // Anaerobe. – 2010. – 16, № 3. – P. 216–225.

  9. Bergey’s Manual of Systematic Bacteriology. 1. The Archaea and the Deeply Branching, and Phototrophic Bacteria / D.R. Boone, R.W. Castenholz, G.M. Garrity, Eds. – 2nd ed. – Springer, 2001. – 721 p.

  10. Взаємодія мікробних популяцій у метаногенних асоціаціях і шляхи збільшення виходу метану в метантенках / В.І. Карпенко, Л.С. Ястремская, Л.П. Голодюк та ін. // Вісник Дніпропетровського ун-ту. Сер. Біологія. Екологія. – 2006. – 1, вип. 14, № 3. – С. 80–85.




DOI: https://doi.org/10.20535/1810-0546.2016.3.53023

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