Agrobacteria as the Potential Producents of Magnetosensitive Nanostructures

Світлана Василівна Горобець, Любов Валеріївна Сорокіна, Тамара Володимирівна Овсієнко


The aim of the study was the identification of the homologues of the Mam proteins mediating the magnetite synthesis of Magnetospirillum gryphiswaldense MSR-1 in the proteome of agrobacteria and their host plants. The identification of the protein homologues was performed by pairwise alignment using the online-service BLAST. It was shown that the strains of symbiotic and pathogenic agrobacteria (AB) that are able for the root nodules formation and their typical host plants could be potential producers contain the homologues of proteins indispensable for magnetite synthesis (MamВ, MamМ, Mamand MamО) in magnetotactic bacteria (MTB). These homologues have a common ancestor, similar folding and common functions with the respective proteins of MTB. Thus, the symbiotic and pathogenic agrobacteria and host plants could be the potential producers of biogenic magnetic nanoparticles or magnetosensitive nanostructures.


Agrobacteria; Magnetotactic bacteria Magnetospirillum gryphiswaldense MSR-1; Proteins of Mam-family


I.E. Dodueva et al., “Plant Tumorigenesis: Different Ways for Shifting Systemic Control of Plant Cell Division and Differentiation”, Transgenic Plant Journal, vol. 1(1), pp. 17–38, 2007.

T. Tzfira and V. Citovsky, Eds., Agrobacterium: From Biology to Biotechnology. New York: Springer, 2008, 735 p.

P.M. Merritt et al., “Motility and chemotaxis in Agrobacterium tumefaciens surface attachment and biofilm formation”, J. of Bacteriol., vol. 189, no. 22, рp. 8005–8014, 2007.

J. Prell and P. Poole, “Metabolic changes of rhizobia in legume nodules”, Trends in Microbiol., vol. 14, no. 4, рp. 161–168, 2006.

D. Amelia et al., “Mechanisms and Regulation of Polar Surface Attachment in Agrobacterium tumefaciens”, Curr. Opin. Microbiol, vol. 12(6), рp. 708–714, Decem¬ber 2009.

A.C. Braun, “Stages in the life history of Phytomonas tumefaciens”, J. Bacteriol., no. 52, рp. 695–702, 1946.

M. Janczarek, “Environmental Signals and Regulatory Pathways That Influence”, Int. J. Mol. Sci., vol. 12, рp. 7898–7933, 2011.

W.S. Wu, “The signaling mechanism of ROS in tumor progression”, Cancer Metastasis Rev., vol. 25(4), pp. 695–705, 2006.

V.F. Chekhun et al., “Magnetic nanostructures in tumour cells”, Research bulletin of the National Academy of Sciences of Ukraine, no. 9, 2011.

V.F. Chekhun et al., “Magnetically ordered nanostructures of endogenous origin in Erlich carcinoma cells”, Nanostructural science of materials (Ukrainian Journal), no. 2, 2011.

Yu.I. Gorobets and S.V. Gorobets, “Stationary flows of electrolytes in the vicinity of ferromagnetic particles in a constant magnetic field”, Bulletin of Kherson State Technical University (Ukrainian Journal), vol. 3(9), pp. 276–281, 2000.

Y.-X. Jing et al., “Effect of magnetic field on symbiotic nitrogen fixation of soybean nodules”, Acta Botanica Sinica, vol. 34, no. 5, pp. 364–368, 1992.

R. Bajwa et al., “Effect of electromagnetism on nodulation, vesicular arbuscular mycorrhizal infection and top growth of chickpea. I. Response of electromagnetized rhizobium”, J. of Phytopathology, vol. 7(1), pp. 76–77, 1995.

M.B. Vainshtein et al., “A new type of magnetosensitive inclusions in cells of photosynthetic purple bacteria”, Syst. Appl. Microbiol, no. 20, pp. 182–186, 1997.

A. Komeili, “Molecular Mechanisms of Compartmentalization and Biomineralization in Magnetotactic Bacteria”, FEMS Microbiol Rev., vol. 36(1), pp. 232–255, 2012.

D. Schuler, “Genetics and cell biology of magnetosome formation in magnetotactic bacteria, FEMS Microbiol. Rev., vol. 32, pp. 654–672, 2008.

Basic Local Alignment Search Tool [Online]. Available:

W. Li et al., “Saturated BLAST: an automated multiple intermediate sequence search used to detect distant homology”, Bioinformatics, vol. 16, no. 12, pp. 1105–1110, 2000.

D. Schüler, “Characterization of the magnetosome membrane in Magnetospirillum gryphiswaldense”, in Biomineralization of nano- and microstructures. Ch. 8, E. Bäuerlein, Ed. Weinheim: Wiley-VCH, 2000, pp. 109–118.

A. Komeili et al., “Magnetosome vesicles are present before magnetite formation, and MamA is required for their activation”, PNAS, vol. 101, no. 11, pp. 3839–3844, 2004.

Uniprot [Online]. Available: uniprot/ F6EAD5

B.Z. Harris and W.A. Lim, “Mechanism and role of PDZ domains in signaling complex assembly”, J. of Cell Science, vol. 114, pp. 3219–3231, 2011.

W. Yang et al., “mamO and mamE genes are essential for magnetosome crystal biomineralization in Magnetospirillum gryphiswaldense MSR-1”, Res. Microbiol., vol. 161, no. 8, pp. 701–705, 2010.

W. Yang et al., “Magnetosomes eliminate intracellular reactive oxygen species in Magnetospirillum gryphiswaldense MSR-1”, Environ. Microbiol., vol. 14, is. 7, pp. 1722–1729, 2012.

O.Yu. Gorobets et al., “Biogenic Magnetic Nanoparticles: Biomineralization in Prokaryotes and Eukaryotes”, Accepted for publication in Dekker Encyclopedia of Nanoscience and Nanotechnology, 3rd ed., 2014.

Чехун В.Ф., Горобець С.В., Горобець О.Ю. та ін. Магніточутливі наноструктури ендогенного походження у клітинах карциноми Ерліха // Наноструктурное материаловед. – 2011. – № 2. – C. 102–109.

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