Allelic Polymorphism Research of Barley Genes Bmy1 and LOX-1 that are Related to Brewing Characteristics of Grain

Антон Андрійович Шаверський, Антон Ігорович Степаненко, Лілія Григорівна Жолнер, Сергій Сергійович Поліщук, Богдан Володимирович Моргун

Abstract


This work was aimed at allelic status identification of the barley genes Bmy1 and LOX-1 in the collection, consisted of 103 domestic and foreign cultivars, and also selection lines. Research of the collection was held using polymerase chain reaction (PCR) with a subsequent analysis of gene LOX-1 allelic polymorphism via restriction of amplification products. Results of the PCR and restriction were visualized using gel-electrophoresis method. It was defined that 63 barley cultivars would be promising for brewing because of their middle activity and thermostability of β-amylase in malt. There was not a single cultivar or selection line with allele loxA, which was responsible for non-active type of lipoxygenase enzyme in beer, in the barley collection. Obtained results indicate low frequency of brewing alleles’ occurrence. Further searching with the help of the newest molecular markers for allelic diversity identification of genes Bmy1 and LOX-1, and, additionally, expansion of the general collection of barley cultivars and selection lines, will allow better differentiation of cultivars and choose among them the most advantageous ones that will become raw material for quality beer.


Keywords


Polymerase chain reaction; Allelic polymorphism, β-amylase, Lipoxygenase-1, Brewing, Barley

References


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DOI: http://dx.doi.org/10.20535/1810-0546.2014.3.52970

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