Allelic Polymorphism Research of Barley Genes Bmy1 and LOX-1 that are Related to Brewing Characteristics of Grain

Authors

  • Антон Андрійович Шаверський Institute of cell biology and genetic engineering of the NASU, Ukraine
  • Антон Ігорович Степаненко Institute of cell biology and genetic engineering of the NASU, Ukraine
  • Лілія Григорівна Жолнер NTUU KPI, Ukraine
  • Сергій Сергійович Поліщук Plant Breeding and Genetics – National Center of Seed & Cultivar Invastigation of the UAAS, Ukraine
  • Богдан Володимирович Моргун Institute of cell biology and genetic engineering of the NASU, Ukraine

DOI:

https://doi.org/10.20535/1810-0546.2014.3.52970

Keywords:

Polymerase chain reaction, Allelic polymorphism, β-amylase, Lipoxygenase-1, Brewing, Barley

Abstract

This work was aimed at allelic status identification of the barley genes Bmy1 and LOX-1 in the collection, consisted of 103 domestic and foreign cultivars, and also selection lines. Research of the collection was held using polymerase chain reaction (PCR) with a subsequent analysis of gene LOX-1 allelic polymorphism via restriction of amplification products. Results of the PCR and restriction were visualized using gel-electrophoresis method. It was defined that 63 barley cultivars would be promising for brewing because of their middle activity and thermostability of β-amylase in malt. There was not a single cultivar or selection line with allele loxA, which was responsible for non-active type of lipoxygenase enzyme in beer, in the barley collection. Obtained results indicate low frequency of brewing alleles’ occurrence. Further searching with the help of the newest molecular markers for allelic diversity identification of genes Bmy1 and LOX-1, and, additionally, expansion of the general collection of barley cultivars and selection lines, will allow better differentiation of cultivars and choose among them the most advantageous ones that will become raw material for quality beer.

Author Biographies

Антон Андрійович Шаверський, Institute of cell biology and genetic engineering of the NASU

Shaverskyi Anton A., first category engineer, student at the NTUU KPI.

Антон Ігорович Степаненко, Institute of cell biology and genetic engineering of the NASU

Stepanenko Anton І., leading engineer, postgraduate student at the NTUU KPI.

Лілія Григорівна Жолнер, NTUU KPI

Zholner Liliya G., candidate of sciences (biology), associate professor, assistant professor  

Сергій Сергійович Поліщук, Plant Breeding and Genetics – National Center of Seed & Cultivar Invastigation of the UAAS

Polishchuk Serhii S., junior research fellow, postgraduate student at the NTUU KPI.

Богдан Володимирович Моргун, Institute of cell biology and genetic engineering of the NASU

Morgun Bogdan V., candidate of sciences (biology), senior research fellow

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Published

2014-06-27