Reserching Electrical Activity of Hippocampal Neurons in Culture

Катерина Анатоліївна Казмірчук, Анастасія Олександрівна Москалюк, Євгеній Васильович Кузьмінський

Abstract


It is shown in this paper that the presynaptic cell directly causes postsynaptic current in the postsynaptic neuron determines its amplitude. Combination of the electrophysiological techniques, mathematical tools, and сytochemical research allow deeper analysis and understanding of the differences in the mechanisms of generation series of action potentials and excitatory inhibitory neurons in the hippocampus. For electrophysiological studies of synaptic interactions involving pairs of cultured hippocampal neurons at different types of pulsed electrical activity of the presynaptic cell the following methodological approaches were used: cooking culture dissociated hippocampal neurons in low density, the method of fixing the intracellular potential on the postsynaptic neuron configuration “whole-cell”; method of pair registration, the method of local extracellular perfusion program for blocking brake and exciting synaptic transmission and selective potassium channel blockers; сytochemical analysis, mathematical methods and processing results. It was also shown that there are two groups of GABA-ergic interneurons in hippocampal culture, which differ by the type of electrical activity: АР is able to generate high-frequency and unable to generate high-frequency capable of generating action potential in response to prolonged stimulation of depolarizing current pulses. These groups significantly differ also by the kinetic properties of АP.

Keywords


Hippocampus; Electrical activity; Action potential; Synaptic transmission; GABA-ergic interneurons; Cultivation; Parvalbumin; Cytochemical painting

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DOI: https://doi.org/10.20535/1810-0546.2014.3.52650

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