Cultivation of Higher Basidiomycetes Schizophyllum Commune on Agar Nutrient Mediums

Authors

  • Ася Сергіївна Бухало M.G. Kholodny Institute of botany, National Academy of Sciences of the Ukraine, Ukraine
  • Віта Михайлівна Ліновицька NTUU KPI, Ukraine

DOI:

https://doi.org/10.20535/1810-0546.2014.3.52204

Keywords:

Biotechnology of higher basidiomycetes, Schizophyllum commune, Agar nutrient mediums, Morphological features of colonies

Abstract

The growth and morphological features of 8 strains of medicinal mushroom Schizophyllum commune Fr. from the Culture collection of Mushrooms of the N.G. Kholodny Institute of Botany National Аcademy of Sciences of Ukraine, including 3 strains dedicated by the authors in different regions of Ukraine, were studied. The researches were investigated on 14 agar media: wort agar, potato‑glucose agar, synthetic medium with L‑asparagine or L‑asparagine with thiamin, medium of Norkrans, Czapek medium, modified Murashige and Skoog medium without hormones, glucose‑pepton‑yeast medium. Effect of some organic compounds may be the main or additional source of carbon, such as casein, carboxymethyl cellulose, pectin, starch and gelatin was determined on pepton‑yeast medium. As a result, comparison of natural and synthetic mediums in terms of their practical use was determined. Most favorable for growth of the majority strains was beer-wort agar, potato‑glucose agar, as well as synthetic medium with asparagine and thiamin. Strains 1590 and 1766 S. commune with high level of radial rate of growth on different mediums were selected for following investigation as the biotechnological and mycology application.

 

Author Biographies

Ася Сергіївна Бухало, M.G. Kholodny Institute of botany, National Academy of Sciences of the Ukraine

Buchalo Asya S., doctor of biological sciences, professor, senior research worker of department of mycology of M.G.Kholodny

Віта Михайлівна Ліновицька, NTUU KPI

Linovytska Vita M., senior teacher of the department of industrial biotechnology of the faculty of biotechnology and bioengineering

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Published

2014-06-27

Issue

No. 3 (2014): Chemical and Biological Sciences and Technologies

Section

Art

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