Cultivation of Higher Basidiomycetes Schizophyllum Commune on Agar Nutrient Mediums

Ася Сергіївна Бухало, Віта Михайлівна Ліновицька

Abstract


The growth and morphological features of 8 strains of medicinal mushroom Schizophyllum commune Fr. from the Culture collection of Mushrooms of the N.G. Kholodny Institute of Botany National Аcademy of Sciences of Ukraine, including 3 strains dedicated by the authors in different regions of Ukraine, were studied. The researches were investigated on 14 agar media: wort agar, potato‑glucose agar, synthetic medium with L‑asparagine or L‑asparagine with thiamin, medium of Norkrans, Czapek medium, modified Murashige and Skoog medium without hormones, glucose‑pepton‑yeast medium. Effect of some organic compounds may be the main or additional source of carbon, such as casein, carboxymethyl cellulose, pectin, starch and gelatin was determined on pepton‑yeast medium. As a result, comparison of natural and synthetic mediums in terms of their practical use was determined. Most favorable for growth of the majority strains was beer-wort agar, potato‑glucose agar, as well as synthetic medium with asparagine and thiamin. Strains 1590 and 1766 S. commune with high level of radial rate of growth on different mediums were selected for following investigation as the biotechnological and mycology application.

 


Keywords


Biotechnology of higher basidiomycetes; Schizophyllum commune; Agar nutrient mediums; Morphological features of colonies

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DOI: https://doi.org/10.20535/1810-0546.2014.3.52204

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