Colloidal Characteristics of Water Systems of Rhamnolipid Biocomplex of Strain Pseudomonas sp. PS-17 with Tween-80 and their Prospects for Biotechnology

Elena V. Karpenko, Vladislav A. Voloshynets, Ilona V. Karpenko, Tetyana Ya. Pokynbroda, Ihor V. Semenyuk, Halyna G. Midyana


Background. Efficiency of mixed systems of biogenic and synthetic surfactants, based on their colloidal chemical properties, in environmentally safe technologies.

Objective. The aim of the paper is the study of the aqueous systems’ colloidal characteristics of the rhamnolipid biocomplex with Twееn-80 nonionic surfactant, as well as the study of the obtained mixtures’ action for the emulsion stabilization and for plant growth stimulation.

Methods. The surface tension of RBC, Tween-80, and their mixture solutions was measured by the Du-Nui method (with a platinum ring). The dependence of the surface tension on the surfactant concentration was determined for mixtures with RBC content, %: 0.0; 11.1; 25.0; 42.9; 66.7; 100. The emulsifying activity of the surfactant mixtures was determined on the emulsification index (E24). The surfactant influence on plants was assessed by their morphometric parameters after presowing seed treatment.

Results. It was found that the obtained mixture behavior, regardless of the surfactant concentration, deviates from the ideal mixture – the deviation is negative, which indicates the predominance of RBC in the surface layer. The shapes of the surface tension curves for different surfactant ratios indicate synergistic effects before and after micelle formation. The optimum ratio in the RBC-Tween-80 system for sunflower growth and sunflower oil emulsification is 2:1.

Conclusions. The peculiarities of colloid-chemical characteristics of RBC and Tween-80 systems are determined, the prospects of their use for increasing efficiency of emulsification and sunflower growth stimulation are determined.


Surface activity; Rhamnolipid biocomplex; Twееn-80; Surfactant systems; Еmulsification; Plant growth stimulation


О.А. Soboleva and М.V. Kryvobokova, “Mixed micelles and adsorption layers of a nonionic surfactant with a cationic (monomeric and dimeric)”, Vestnik Moskovskogo Universiteta. Ser. 2. Himiya, vol. 45, no. 5, pp. 344–350, 2004 (in Russian).

H.M. Holland and D.N. Rubi, “Mixed surfactant systems”, in ACS Symp. Ser., vol. 501. Washington: Amer. Chem. Soc., 1992. doi: 10.1021/bk-1992-0501.ch001

M.J. Rosen, “Phenomena in mixed surfactant systems”, in ACS Symp. Ser., vol. 311. Washington: Amer. Chem. Soc., 1986.

А.P. Hrabarovs'ka et al., “Colloidal properties of synthetic emulsifiers and rhamnolipid”, Voprosy Himii i Himicheskoy Tehnologii, no. 6, рp. 149–152, 2008 (in Ukrainian).

A. Harlov et al., “Properties of mixed monomolecular layers of bovine serum albumin and nonionic surfactant Tween-80”, in Proc. MCVI, 2007, pp. 54–64 (in Russian).

C. Prieto and L. Calvo, “Performance of the biocompatible surfactant Tween 80, for the formation of microemulsions suitable for new pharmaceutical processing”, J. Appl. Chem., vol. 2013, 10 p., 2013. doi: 10.1155/2013/930356

O.V. Karpenko et al., “Colloid-chemical characteristics of the products of biosynthesis of Pseudomonas sp. PS-17”, Voprosy Himii i Himicheskoy Tehnologii, no. 2, рp. 30–34, 2012 (in Russian).

I.E. Kłosowska-Chomiczewska et al., “Rhamnolipid CMC prediction”, J. Colloid Interf. Sci., vol. 488, рp. 10–19, 2017. doi: 10.1016/j.jcis.2016.10.055

D.P. Sachdev and S.S. Cameotra, “Biosurfactants in agriculture”, Appl. Microbiol. Biotechnol., vol. 97, pp. 1005–1016, 2013. doi: 10.1007/s00253-012-4641-8

P.A. Brocklehurst and J. Dearman, “A comparison of different chemicals for osmotic treatment of vegetable seed”, Annals Appl. Biology, vol. 105, pp. 391–398, 1984. doi: 10.1111/j.1744-7348.1984.tb03064

F.M. Humpherson-Jones, “Effect of surfactants and fungicides on clubroot (Plasmodiophora brassicae) of brassicas”, Annals Appl. Biology, vol. 122, pp. 457–465, 1993. doi: 10.1111/j.1744-7348.1993.tb04049.x

I.V. Karpenko et al., “Influence of biogenic surfactants on the oilseed growth”, Bulletin of Lviv Polytechnic National University, Chemistry, Technology of Substances and their Application, no. 787, pp. 254–257, 2014 (in Ukrainian).

O.V. Karpenko et al., “Growth stimulation of Gramineae plants by rhamnolipid surfactants”, Biotechnologia Acta, no. 6, pp. 94–99, 2013 (in Ukrainian).

O.V. Karpenko et al., “Biogenic rhamnolipid surfactants in complex plant growth regulators”, Naukovi Visti NTUU KPI, no. 3, pp. 36–41, 2016. doi: 10.20535/1810-0546.2016.3.65576 (in Ukrainian).

I.V. Каrpenko et al., “Prospects of rhamnolipid surfactants in technologies for sunflower growing”, Bulletin of Lviv Polytechnic National University, Chemistry, Technology of Substances and their Application, no. 841, pp. 164–168, 2016 (in Ukrainian).

Y.A. Batta, “Invert emulsion: Method of preparation and application as proper formulation of entomopathogenic fungi”, MethodsX, vol. 3, рp. 119–127, 2016. doi: 10.1016/j.mex.2016.02.001

Agricultural Crop Seeds. Methods for Determining Quality, Ukraine State Standard 4138–2002, 2003 (in Ukrainian).

Y.P. Adler et al., Experiment Planning in the Search for Optimal Conditions. Moscow, SU: Nauka, 1976 (in Russian).

V.V. Nyzhnyk et al., Colloid Chemistry with Elements of Nanochemistry. Kyiv, Ukraine: Fitosotsiotsentr, 2012 (in Ukrainian).

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