Solid Phase Extraction of Trace Amounts of Chloramphenicol on the Silica Gel Surface Modified with Octadecyl Groups

Authors

DOI:

https://doi.org/10.20535/1810-0546.2016.3.69972

Keywords:

Chloramphenicol, Solid phase extraction, Honey, Sorption concentration, ELISA

Abstract

Background. Chloramphenicol (CAP) is a broad spectrum antibiotic. It’s widely used in various fields of veterinary and can be accumulated in food of animal origin. A minimum required performance limit (MRPL) for CAP in food is significantly restricted in the EU and the USA. Most modern methods make it difficult to determine the trace amounts of the drug from various matrices without concentration. The method of liquid-liquid extraction (LLE) may be accompanied by loss of trust component. It is therefore advisable to use solid phase analyte extraction to increase the determination sensitivity by instrumental methods and CL withdrawal stability.

Objective. Development of the solid phase extraction method of chloramphenicol from honey, followed by enzyme linked immunoassay.

Methods. Quantitative determination of chloramphenicol was determined using enzyme linked assay (ELISA) test-kit for determination of chloramphenicol in foods, provided by LLC “XEMA”. Solid phase extraction of chloramphenicol from honey samples was studied on silica gel surface modified with octadecyl groups (SiO2-C18), “Agilent”. This method of solid phase extraction was carried out on samples of honey, that previously checked by ELISA using LLE for sample preparation.

Results. Our results showed the possibility of solid phase extraction of CAP on the surface of the silica gel with oktadecyl groups. The optimal conditions for extraction the analyte on the surface of sorbent were found and the eluation condition for CAP was studied. We tested the method of solid phase extraction of CAP from honey matrics on control materials – honey samples with known concentrations of CAP, that were bought by LLC “XEMA”.

Conclusions. Solid phase extraction of CAP could be used for CAP extraction form honey. The proposed method of sample preparation reduces the time of preparation for honey samples. This method is perspective for developing the method of simultaneous concentration of antibiotics from different groups followed by ELISA determination.

Author Biographies

Ольга Михайлівна Іванова, Taras Shevchenko National University of Kyiv; “ХЕМА” LLC, Kyiv

Olga M. Ivanova,

PhD-student at Taras Shevchenko National University of Kyiv, Head of QC department at LLC “XEMA”

Таїсія Володимирівна Сіколенко, Taras Shevchenko National University of Kyiv; “ХЕМА” LLC, Kyiv

Taisia V. Sikolenko,

masters of analytical chemistry at Taras Shevchenko National University of Kyiv, QC manager at LLC “XEMA”

References

D.C. Plumb, Veterinary Drug Handbook, 4th ed. Ames: Iowa State Press, 2002, pp. 166–169.

N.S. Egorov, Basis of the Doctrine of Antibiotics, 4th ed. Moscow: Vysshaja Shkola, 1986 (in Russian).

Codex Alimentarius Commission Maximum Residue Limits for Veterinary Drugs in Foods Updated as at the 35th Session of the Codex Alimentarius Commission (July, 2012) CAC/MRL 2-2012 [Online]. Available: http://www.codexalimentarius.org/

input/download/standards/45/MRL2_e.pdf

Commission decision of 13 March 2003 amending Decision 2002/657/EC as regards the setting of minimum required per­formance limits (MRPLs) for certain residues in food of animal origin (2003/181/EC), Official J., L 071, pp. 0017–0018, 15.03.2003.

E.H. Allen, “Review of chromatographic methods for chloramphenicol residues in milk, eggs, and tissues from food-pro­ducing animals”, J. Assoc. Off. Anal. Chem., 1985, vol. 68, no. 5, pp. 990–999.

D. Arnold and A. Somogyi, “Trace analysis of chloramphenicol residues in eggs, milk, and meat: comparison of gas chro­ma­to­graphy and radioimmunoassa”, J. Assoc. Off. Anal. Chem., 1985, vol. 68, no. 5, pp. 984–990.

J.F. Huang et al., “Chloramphenicol extraction from honey, milk, and eggs using polymer monolith microextraction followed by liquid chromatography-mass spectrometry determination”, J. Agric. Food. Chem., 1985, vol. 68, no. 5, pp. 984–990.

L. Penney et al., “Determination of chloramphenicol residues in milk, eggs, and tissues by liquid chromatography/mass spectro­metry”, J. AOAC Int., 2005, vol. 88, no. 2, pp. 645–653.

K. Fujita et al., “Determination of chloramphenicol residues in bee pollen by liquid chromatography/tandem mass spectro­metry”, J. AOAC Int., 2008, vol. 91, no. 5, pp. 1103–1109.

A.Y. Kolosova et al., “Competitive ELISA of chloramphenicol: influence of immunoreagent structure and application of the me­thod for the inspection of food of animal origin”, J. Food Agricul. Immun., 2000, vol. 12, no. 2, pp. 115–125.

S. Impens et al., “Screening and confirmation of chloramphenicol in shrimp tissue using ELISA in combination with GC–MS and LC–MS”, J. Analyt. Chim. Acta, 2003, vol. 483, no. 1-2, pp. 153–163.

H.Y. Shen and H.L. Jiang, “Screening, determination and confirmation of chloramphenicol in seafood, meat and honey using ELISA, HPLC–UVD, GC–ECD, GC–MS–EI–SIM and GCMS–NCI–SIM methods”, J. Analyt. Chim. Acta, 2005, vol. 535, pp. 33–41.

Published

2016-06-30