Selection of ‎Methods for Carotenoid Extraction from Submerged Laetiporus sulphureus Mycelium

Authors

DOI:

https://doi.org/10.20535/1810-0546.2016.3.60515

Keywords:

Carotenoids, Xanthоcarotene, Extraction, Optical density, Submerged cultivation, Laetiporus sulphureus

Abstract

Background. To obtainnatural carotenoids for their practical application is the actual problem. Xanthоcarotene obtained through biotechnology can be used in light industry as dye, as an alternative to artificial pigments. The need of dedication of natural carotenoidsis also caused by their suitability for medical use. It is explained by diverse biological activity (antitumor, radioprotective, antimicrobial, antiviral, antioxidant) of these substances.

Objective. Objective of the study is selection of effective methods and conditions of carotenoid extraction from Laetiporus sulphureus biomass.

Methods. After the submerged producer’s cultivation was performed different methods of pigments extraction were carried out. Carotenoid content was determined.

Results. The most effective extraction was chosen. Carotenoid content in producer’s biomass was calculated.

Conclusions. Еthanol-hexane method was selected as the most effective extraction circuit. Also was found that the change of exposure’s temperature causes changes in the composition of the key faction. 

Author Biographies

Лариса Петрівна Дзигун, NTUU ''KPI''

Larysa P. Dzyhun,

senior lecturer at the Industrial Biotechnology Department of Biotechnology and and Biotechnics Faculty

Оксана Анатоліївна Палюшок, Magen David Adom in Israel

Oksana A. Palushok,

laboratory assistant at the National blood services centr, Вachelor of the Industrial Biotechnology Department of the National Technical University "Kyiv Polytechnic Institute"

Олександра Миколаївна Чуднівець, Shupyk National Medical Academy of Postgraduate Education

Oleksandra M. Chudnivets,

senior laboratory assistant at the Virology Department, student at the Industrial Biotechnology Department of the National Technical University "Kyiv Polytechnic Institute"

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Published

2016-06-30