Cultivation of Higher Basidiomycetes Schizophyllum Commune on Agar Nutrient Mediums

Authors

  • Ася Сергіївна Бухало M.G. Kholodny Institute of botany, National Academy of Sciences of the Ukraine, Ukraine
  • Віта Михайлівна Ліновицька NTUU KPI, Ukraine

DOI:

https://doi.org/10.20535/1810-0546.2014.3.52204

Keywords:

Biotechnology of higher basidiomycetes, Schizophyllum commune, Agar nutrient mediums, Morphological features of colonies

Abstract

The growth and morphological features of 8 strains of medicinal mushroom Schizophyllum commune Fr. from the Culture collection of Mushrooms of the N.G. Kholodny Institute of Botany National Аcademy of Sciences of Ukraine, including 3 strains dedicated by the authors in different regions of Ukraine, were studied. The researches were investigated on 14 agar media: wort agar, potato‑glucose agar, synthetic medium with L‑asparagine or L‑asparagine with thiamin, medium of Norkrans, Czapek medium, modified Murashige and Skoog medium without hormones, glucose‑pepton‑yeast medium. Effect of some organic compounds may be the main or additional source of carbon, such as casein, carboxymethyl cellulose, pectin, starch and gelatin was determined on pepton‑yeast medium. As a result, comparison of natural and synthetic mediums in terms of their practical use was determined. Most favorable for growth of the majority strains was beer-wort agar, potato‑glucose agar, as well as synthetic medium with asparagine and thiamin. Strains 1590 and 1766 S. commune with high level of radial rate of growth on different mediums were selected for following investigation as the biotechnological and mycology application.

 

Author Biographies

Ася Сергіївна Бухало, M.G. Kholodny Institute of botany, National Academy of Sciences of the Ukraine

Buchalo Asya S., doctor of biological sciences, professor, senior research worker of department of mycology of M.G.Kholodny

Віта Михайлівна Ліновицька, NTUU KPI

Linovytska Vita M., senior teacher of the department of industrial biotechnology of the faculty of biotechnology and bioengineering

References

Ch. Hobbs, “Medicinal Mushrooms: An exploration of Tra­dition, Healing and Culture,” Santa Cruz: Botanica Press, 1995, 251 p.

S.P. Wasser et al., “Medicinal Mushrooms: Past, Present and Future,” Ukr. Bot. J., vol. 5, pp. 499–524, 2002.

Биологические свойства лекарственных макромицетов в культуре: Сб. науч. трудов в 2 т. / Под ред. чл.-кор. НАН Украины С.П. Вассера; Ін-т ботаніки ім. М.Г. Холодного НАН України. – К.: Альтепрес, 2012. – 459 с.

A. Mansour et al.,Schizophyllan inhibits the development of mammary and hepatic carcinomas induced by 7,12 dimethylbenz(α)anthracene and decreases cell proliferation: comparison with tamoxifen”, J. Cancer Res. Clin. Oncol., vol. 138, no. 9, pp. 1579–1596, 2012.

H.H. Arbaayah and Y.U. Kalsom, “Antioxidant properties in the oyster mushrooms (Pleurotus spp.) and split gill mushroom (Schizophyllum commune) ethanolic extracts”, Mycosphere, vol. 4, no. 4, pp. 661–673, 2013.

Бухало А.С. Высшие съедобные базидиомицеты в чистой культуре. – К.: Наук. думка, 1988. – 144 с.

Методы экспериментальной микологии: Справочник / И.А. Дудка, С.П. Вассер, И.А. Элланская и др. – К.: Наук. думка, 1982. – 561 с.

Мусієнко М.М., Панюта О.О. Біотехнологія рослин. Навч. посібник. – К.: ВПЦ “Київський уні­вер­ситет”, 2005. – 114 с.

Сатон Д., Фотергилл А., Ринальди М. Определитель патогенных и условно патогенных грибов / Пер. с англ. – М.: Мир, 2001. – 486 с.

Цизь А.М., Бисько Н.А. Рост мицелия лекарственных грибов порядка Aphyllophorales на различных питательных средах // Успехи мед.

микологии. – М.: Нац. академия микологии, 2007. – Т. 9 – C. 266–268.

N. Sutivisedsak et al., “Production of schizophyllan from distiller’s dried grains with solubles by diverse strains of Schizophyllum commune”, SpringerPlus, vol. 2, p. 476, 2013.

I.S. Mohd Kamal et al., “Optimization of media for mass production of Schizophyllum commune using response surface methodology (RSM)”, in Proc. MICOTriBE, pp. 1–8, 2012.

Y.P. Teon and M. Mat Don, “In vitro Antifungal Ac­ti­vi­ties and Phytochemical Analysis of Filamentous White-rot Fungi, Schizophyllum commune”, Sains Malaysiana, vol. 42, no. 9, pp. 1267–1272, 2013.

Published

2014-06-27